LOCUS       sequence-120134-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DdMCU-Flag in pACT2 vector human codon optimised.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber
            RJ, Myre MA, Blower MD, Mootha VK
  TITLE     Reconstitution of the mitochondrial calcium uniporter in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 
            10.1073/pnas.1400514111. Epub 2014 Jun 2.
  PUBMED    24889638
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400514111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8985-90"
COMMENT     Sequence Label: DdMCU
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    251..391
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(317..336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     477..499
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        528..929
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
       61 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      241 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      301 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
      361 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
      421 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      481 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcaacttct
      541 tttctttttt tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa
      601 aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg
      661 ttgttccaga gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc
      721 acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg
      781 aaataaaaaa aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct
      841 tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat
      901 ttcaagctat accaagcata caatcaactc caagcttgat gaatagcttc gtgatcagga
      961 acggcttcgg cctcgtcagg accttcaaca ccaggctgtt taccacctcc acccagaacc
     1021 tggagggcga gctcaaaacc atcctcggcc aggccaaggt cagcaagctg caggaaa
//
