LOCUS       sequence-120140-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CALR
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gaacagctcg tccttggcct ggccggggac atcttcctcc tcatcttcct
      241 ccttgtcctc ctcatcctcc tcatcctcat ctttgtcctc atcatcctcc ttgtcctctg
      301 cctcctcctc ctctttgcgt ttcttgtctt cttcctcctc cttaagcctc tgctcctcgt
      361 cctgtttgtc cttcatttgt ttctctgctg cctttgttac gccccacgtc tcgttgccaa
      421 actcctcagc gtatgcctca tcgttggtga tgaggaagtt gtcaaagatg gtgccagact
      481 tgacctgcca gaggtccagg cccagcacgc caaagttatc ataggcatag atactgggat
      541 cgggagaata ctcggggttg tcaatttctg ggtggatcca agtgcccttg taatctgggt
      601 tgtcgatctg ccggggcttc cactcaccct tgtactcagg gttctgaatc actgggggtt
      661 cccactctcc gtccatctct tcatcccagt cctcgggctt cttagcatca gggtcaggga
      721 tatgctcggg cttgtcccag tcctcaggct tggagtctgt gggatcatcg atcttggccc
      781 gctcatccca gtcttccggt tttgaagcat
//
