LOCUS       sequence-120144-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DdMCU-Flag in pACT2 vector human codon optimised.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber
            RJ, Myre MA, Blower MD, Mootha VK
  TITLE     Reconstitution of the mitochondrial calcium uniporter in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 
            10.1073/pnas.1400514111. Epub 2014 Jun 2.
  PUBMED    24889638
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400514111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8985-90"
COMMENT     Sequence Label: DdMCU
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(184..207)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta gaagtgtcaa
       61 caacgtatct accaacgatt tgaccctttt ccatcttttc gtaaatttct ggcaaggtag
      121 acaagccgac aaccttgatt ggagacttga ccaaacctct ggcgaagaag tccaaagctt
      181 ttacttgtca tcgtcatcct tgtaatccat agatccacca gatccaccag atccaccccc
      241 ggggtgggtg tgatcgtact ttgtggcgag ctcgagctcc ttgagctcct tctccttctt
      301 gtcgatggcc tgcacgaggt tctcgtagtc ctccttaggg aagttgtttc ttttgaacag
      361 cttgtcctgc ctcttgctga acagcctatg gttgagagcc tcgtaggtga actcggtctt
      421 ggtcatggtg aagtaggtgt agccgatcag gacggagccg aaggtcagga agtagctcac
      481 gggctcgatg atgtcccagg agaggtccca ccaggtgagc ctagccagaa tggcggcctg
      541 ggcgaagcaa tagccgagtc cggtccagat gatagcggtg gccctcctgt gggcctttct
      601 gtcgatcacc tgcttcttct cctccagggg ctggatcttc tgcctcaggc tattgatctc
      661 ggatttcttg gactcgatca gcttattcag gcccacgccc ttgttctcga tgtccaggat
      721 gtgttcgagg gactggtaga tatgggcggg cttggtgaac acggaggtct tcaggttctc
      781 gttcagggag ttaggcaggt agatgatgga gccggattga gccagggcgt tgctgacgct
      841 gttgatttcc ttctcctcga tgcccacctc cttggcgatg cccttgaagt cgttgaaggt
      901 gatcttggac ctgggatcca gcttcagctt ttcctgcagc ttgctgacct tggcctggcc
      961 gaggatggtt ttgagctcgc cctccaggtt ctgggtggag gtggtaaa
//
