LOCUS       sequence-120146-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PSMC1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtagagatac agcccctcag gggtgccttc ctgtttctta taaagaacat
      241 tttctttaga ttttttgaag tcttcatttg ttactttcat tctacgttct cttaaggcca
      301 tcagaccagc ttctgtacag attgccttga tgtcagcacc agagaggtca tctttagcca
      361 tgatcaggtc gtccagggtt acatcatcag ccagcgtcat cctgcttgtg tgaatctgaa
      421 agatgcgctt cttcgtcttt tcatcaggca gggggaactc aatcttcctg tcaatgcggc
      481 ctggtctgat aagtgctgga tccaaagttt ctattcggtt tgtggccatg ataactttca
      541 catctcccct agaatcaaat ccatccaact ggttcagcag ttccaacatt gttcgctgaa
      601 tttctctctc accaccagaa ttggagtcat atccttttgt cccaatggcg tcaacttcat
      661 caataaacac gatggacggt gcatgttctc cagcaactcg gaacaattcc cgtacgagtt
      721 tgggcccatc acctaggtac ttctgaataa gttcagagcc aaccactctc aagaaagtgg
      781 ctgaggtttg gtttgctact gctttggcta acaaggtttt acctgtgcca ggtggaccat
      841 agagaatgac ccccttagga ggctttatac ccatctcttc ataatattca ggatggggtg
//
