LOCUS       sequence-120162-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: MRGPRX4-Tango
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga aacccacctg cttactgggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat
      121 ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgcgccacca
      181 tgaagacgat catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg
      241 atgatgacgc cagcatcgat atggatccta ctgtgcctgt gtttggtaca aagctcaccc
      301 ctattaacgg gagagaggaa actccctgct ataatcagac cctctctttc accgtgctca
      361 catgtatcat cagtctggtc gggctgacag gcaacgcagt ggtactttgg ttgctggggt
      421 atagaatgcg acgcaatgcc gtgagtattt atatccttaa tttggcagcc gcagattttc
      481 tgtttctctc cttccaaatc atccgcctgc ctctgcgact cattaatatc tctcacctta
      541 ttcggaagat cctcgtgtca gtgatgacat ttccctattt cacaggtctg agcatgctct
      601 cagccattag caccgaacgg tgtttgagtg tcctgtggcc catatggtac cgatgcagaa
      661 gacccactca cctgtccgca gtggtttgtg tgcttctgtg gggcctgtca ctgctgttct
      721 ccatgctgga atggcgcttc tgtgactttc tcttctccgg agctgactcc agctggtgcg
      781 agacctccga ttttattccg gtcgcttggc tcatctttct ttgtgtagta ctttgtgtgt
      841 catccttggt actgctcgtc agaattctgt gtgggtccag aaagatgccg ctcacacggt
      901 tgtacgtcac aattttgctc acagttc
//
