LOCUS       sequence-120163-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DdMCU-Flag in pACT2 vector human codon optimised.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber
            RJ, Myre MA, Blower MD, Mootha VK
  TITLE     Reconstitution of the mitochondrial calcium uniporter in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 
            10.1073/pnas.1400514111. Epub 2014 Jun 2.
  PUBMED    24889638
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400514111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8985-90"
COMMENT     Sequence Label: DdMCU
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    509..542
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    620..653
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    697..730
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    808..841
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    885..918
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    996..1029
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaagattctc tttttttatg atatttgtac ataaacttta taaatgaaat tcataataga
       61 aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat
      121 ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat
      181 tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt aatattgaca
      241 aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac tatgattcct
      301 aatttatata ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc
      361 aatgacctga ccatttgatg gagtttaagt caataccttc ttgaaccatt tcccataatg
      421 gtgaaagttc cctcaagaat tttactctgt cagaaacggc cttaacgacg tagtcgacca
      481 attccgatca tattcaataa cccttaatat aacttcgtat aatgtatgct atacgaagtt
      541 attaggtctg aagaggagtt tacgtccagc caagctagct tggctgcagg tcgagcggcc
      601 gcgatccgga acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc
      661 ctcgaccaat tccgatcata ttcaataacc cttaatataa cttcgtataa tgtatgctat
      721 acgaagttat taggtctgaa gaggagttta cgtccagcca agctagcttg gctgcaggtc
      781 gagcggccgc gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
      841 ttaggtccct cgaccaattc cgatcatatt caataaccct taatataact tcgtataatg
      901 tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag ctagcttggc
      961 tgcaggtcga gcggccgcga tccggaaccc ttaatataac ttcgtataat gtatgctata
     1021 cgaagttatt agg
//
