LOCUS       sequence-120165-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: NPFF1-Tango
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactggc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      121 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg cgccaccatg
      181 aagacgatca tcgccctgag ctacatcttc tgcctggtat tcgccgacta caaggacgat
      241 gatgacgcca gcatcgatat ggagggggaa ccctcacagc ctccgaactc ttcctggcca
      301 cttagccaga acggcacaaa taccgaggcc acccctgcca cgaatctgac tttcagctca
      361 tactaccagc acaccagccc tgtggccgct atgttcatag tggcctatgc ccttatcttc
      421 cttctgtgta tggtaggaaa caccctggtg tgttttatcg tccttaaaaa ccgccatatg
      481 cacaccgtga ctaacatgtt tatcctcaac ctggccgtct ctgatcttct cgtggggatc
      541 ttctgcatgc ctacaactct ggtggataat ttgataactg gatggccttt tgataatgct
      601 acctgtaaga tgtctggtct cgtacaaggg atgagtgtgt ctgcttctgt gtttactctc
      661 gtggcgattg cggtagagag attcaggtgt atcgtgcacc cattcaggga aaaactgaca
      721 ctgaggaagg cccttgtaac catcgcagta atttgggcac tggctctgct catcatgtgt
      781 cctagtgcag tcaccctcac cgtaacaagg gaggagcacc acttcatggt cgacgccaga
      841 aatcgaagct atcctctgta tagttgttgg gaggcatggc cagagaaagg tatgcgccgc
      901 gtgtacacaa ctgtcttg
//
