LOCUS       sequence-120180-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Create a docking site in the mouse TIGRE genomic locus that allows 
            modification in ES cells by Flp recombinase-mediated cassette 
            exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: TIGRE docking site targeting vector
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..75
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    complement(169..202)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
ORIGIN
        1 gcgcctcccc tacccggtag aattcgataa atgcatccaa acgcgcccgg ggagcccaag
       61 ggcacgccct ggcacctaaa acatatggct tctgcaataa gatctatgcc aagcttttac
      121 ggcgcgccag aataagtcgt taaatttaaa tccgaaggcc ggccgaaaga agttcctata
      181 ctatttgaag aataggaact tcgcacagga tccaagaatt cctgagctca aagcttactt
      241 ctagaggtcg acctttaatt ctagcgatct ggaggcagag gcaggtggat ctatgagttt
      301 gaggctggct tgctctacac agtgagttcc agaaaagcca gggctacaca gtgagaccct
      361 atctagaaac aacaacagca acaaaaaata tacacatctc tcctaaaaat aaaacacgca
      421 gtctggaagt aagtgaccgt tggggaagca ctggaaagga gttagagaat gtagacctct
      481 tgtgcctgac tgggtgtccg gattcaatag tgacttctcc ggtgatctgg gaatctgtga
      541 agaggttctg gctctctgtc tagcctgagg ctgatacccc aactcatttt cttgtcttct
      601 gtgaggaagg ttacaactct aggagtcccc ttcctttggc cagtgcttcc ctaagaaagc
      661 tatggcttga ttcccacccc caccctacct ccactgccaa gtctctggac agatgtgcag
      721 cctctggcag t
//
