LOCUS       sequence-120219-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target a Cre-dependent GCaMP6-fast expression cassette to the mouse 
            Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Ai95(RCL-GCaMP6f) targeting vector
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..64)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    141..189
                     /note="synthetic polyadenylation signal"
     polyA_signal    198..674
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(397..416)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gatttgatac cgcgggcccg tattcgaaat ataacttcgt atagcataca ttatacgaag
       61 ttattcgcga tgaataaatg aaagcttgca gatcccctca gcaccatggc tagcggcagc
      121 ctcggagttt gaatagatag aataaaatat ctttattttc attccatctg tgtgttggtt
      181 ttttgtgtga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
      241 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
      301 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
      361 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
      421 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
      481 ctcaggctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
      541 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
      601 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
      661 ccttccctgt ccttctgcct cagcgctgta agtctgcaga aattgatgat ctattaa
//
