LOCUS       sequence-120221-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-eBFP2
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(493..514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
       61 agctcaccgt ctttcattgc catacggaat tcgagctcgc acgctgccgc aagcactcag
      121 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
      181 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
      241 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
      301 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
      361 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctt
      421 gactagaaat aagaaggaga tataattatg gtgagcaagg gcgaggagct gttcaccggg
      481 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgagg
      541 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
      601 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgagccacgg cgtgcagtgc
      661 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      721 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcacctacaa gacccgcgcc
      781 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg cgtcgacttc
      841 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaa
//
