LOCUS       sequence-120229-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-bjGFP
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(479..500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(740..759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
       61 tttcattgcc atacggaatt cgagctcgca cgctgccgca agcactcagg gcgcaagggc
      121 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
      181 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
      241 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
      301 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
      361 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatcttg actagaaata
      421 agaaggagat ataattatgt cgaagggcga ggagctgttc accggcgtcg tcccgatcct
      481 ggtcgagctg gacggcgacg tcaacggcca caagttctcg gtctcgggcg agggcgaggg
      541 cgacgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgccggt
      601 cccgtggccg accctggtca ccaccctgac ctacggcgtc cagtgcttct cgcgctaccc
      661 ggaccacatg aagcgccacg acttcttcaa gtcggccatg ccggagggct acgtccagga
      721 gcgcaccatc tcgttcaagg acgacggcaa ctacaagacc
//
