LOCUS       sequence-120265-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target a Dre and Cre-dependent tdTomato expression cassette to the 
            mouse Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Ai66(RCRL-tdT) targeting vector
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..60
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    106..154
                     /note="synthetic polyadenylation signal"
     polyA_signal    163..639
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(362..381)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gatttgatac cgcgggcccg tattcgaata actttaaata atgccaatta tttaaagtta
       61 cctcagcacc atggctagcg gcagcctcgg agtttgaata gatagaataa aatatcttta
      121 ttttcattcc atctgtgtgt tggttttttg tgtgagatct acgggtggca tccctgtgac
      181 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
      241 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
      301 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
      361 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
      421 tcctgggttc aagcgattct cctgcctcag gctcccgagt tgttgggatt ccaggcatgc
      481 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
      541 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
      601 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgcctcagct aattgagtag
      661 gg
//
