LOCUS       sequence-120269-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PSMC1
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..54)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(198..217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(199..217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        249..353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             538..916
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(572..591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cccggggacg ctctcgccct tgcttgtgcc gtggtgatgg tggtgatggt ggtgaccaga
       61 ggtgcccatg gttttcactt acgtgtttaa tgtctttaca gactttatac attactgtca
      121 tggttaaata aaataaaata aaataaaata aaataaaata aaataaaata aaataaaata
      181 aaataaactt aagatgtccc tatagtgagt cgtattagac gtcaggtggc acttttcggg
      241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      961 aacaattaat agactggatg gaggcggata agttgcagga cacttctgcg ctcggccctt
     1021 ccggctggct gggttatggc
//
