LOCUS       sequence-12027-e        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPN8
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..127
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1129..1146
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggttccgcg
      121 tggtagtaac gctttgaagt acttgggcca ggatttcaag accctgaggc aacagtgctt
      181 ggactcaggg gtcctattta aggaccctga gttcccagca tgtccatcag ctttgggcta
      241 caaggatctt ggaccaggct ctccgcaaac tcaaggcatc atctggaagc ggcccacgga
      301 gttgtgtccc agccctcagt ttatcgttgg tggagccacg cgcacagaca tttgtcaggg
      361 tggtctaggt gactgctggc ttctggctgc cattgcctcc ctgaccctga atgaagagct
      421 gctttaccgg gtggtcccca gggaccagga cttccaggag aactatgcgg gaatctttca
      481 ctttcagttc tggcagtacg gagagtgggt ggaggtggtc attgacgaca ggctgcccac
      541 caagaatgga cagctgctct tcctacactc ggaacaaggc aatgaattct ggagtgccct
      601 gctggagaaa gcctatgcca agcttaatgg ttgttatgag gctctcgctg gaggttccac
      661 agtggagggg tttgaggatt tcacaggtgg catctctgag ttttatgacc tgaagaaacc
      721 accagccaat ctatatcaga tcatccggaa ggccctctgt gcggggtctc tgctgggctg
      781 ctccattgat gtctacagtg cagccgaagc cgaagccatc accagccaga agctggttaa
      841 gagtcatgcg tactctgtca ctggagtcga agaggtgaat ttccagggcc atccagagaa
      901 gctgatcaga ctcaggaatc catggggtga agtggagtgg tcgggagcct ggagcgatga
      961 tgcaccagag tggaatcaca tagacccccg gcggaaggaa gaactggaca agaaagttga
     1021 ggatggagaa ttctggatgt cactttcaga tttcgtgagg cagttctctc ggttggagat
     1081 ctgcaacctg tccccggact gatgacgaag cttgcggccg cactcgagca ccaccaccac
     1141 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgcca
//
