LOCUS       sequence-120272-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pCS-Fv2Eflt4-2a-tdTomato
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             52..93
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             100..420
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             427..747
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tcgattcgaa ttcaaggatc aacaagtttg tacaaaaaag caggctggac catggggagt
       61 agcaagagca agcctaagga ccccagccag cgctctagag gcgtccaagt cgaaaccatt
      121 agtcccggcg atggcagaac atttcctaaa aggggacaaa catgtgtcgt ccattataca
      181 ggcatgttgg aggacggcaa aaaggtggac agtagtagag atcgcaataa acctttcaaa
      241 ttcatgttgg gaaaacaaga agtcattagg ggatgggagg agggcgtggc tcaaatgtcc
      301 gtcggccaac gcgctaagct caccatcagc cccgactacg catacggcgc taccggacat
      361 cccggaatta ttccccctca cgctaccttg gtgtttgacg tcgaactgtt gaagctcgag
      421 actagaggag tgcaggtgga gactatctcc ccaggagacg ggcgcacctt ccccaagcgc
      481 ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc
      541 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
      601 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
      661 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
      721 ttcgatgtgg agcttctaaa actggaaact agagtgaatg tcaagcgagt gaacccagct
      781 gatatcaaga caggctatct gtccatcatc atggacccgg gggaagtgcc gctggaggag
      841 cagtgtgaat acctgccgta cgactccagc cagtgg
//
