LOCUS       sequence-120273-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFDP2
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtattctggg gaggaggaat cctcctcatc atcttcctca tcttcatcat
      241 tgaacgaaca gggggtctcg cctcgggact cggagcagtg agaggccgca ctgctggact
      301 ggtgactgtt tggggccagg aactgcccag ttgctaaggc cacttctgca tccaagcata
      361 acccttggtt tacacttgac tggggtaagg tggcaccagt ggtcaggtct aaatttgaaa
      421 ctgattgggt agagttcaga agtagtccct gatttaacca agaaggtcct gtggagatat
      481 ctgtgatata accttctaaa gcctttggca ccagggattt cgcaagtttc agatcctcca
      541 gagagcattt gcctgactcc aggccaaacg acattcccat ccgctttagt acttctatgt
      601 catcatggat ctcaaaggtg ttgtcaaaat tgaaaagata ctcaaacttg tcactggaga
      661 tgctgcaatc tatgactgtt tttctgcttg tattgatgat tatgaatggc agctgaatgg
      721 tagagttcag agccggcggg ccctggtttt gctgctcatt ttgtcgattt ctctgtacca
      781 ggtttttgaa agcgatttgc tgtaggagaa gttcttgcag ctgggcccgc ttctgcttta
      841 tccgttctat ccgcctctgc ttctctatct ccagattctg acattcctga gcagaattgg
//
