LOCUS       sequence-120277-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TNF
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcacagggca atgatcccaa agtagacctg cccagactcg gcaaagtcga
      241 gatagtcggg ccgattgatc tcagcgctga gtcggtcacc cttctccagc tggaagaccc
      301 ctcccagata gatgggctca taccagggct tggcctcagc cccctctggg gtctccctct
      361 ggcaggggct cttgatggca gagaggaggt tgaccttggt ctggtaggag acggcgatgc
      421 ggctgatggt gtgggtgagg agcacatggg tggaggggca gccttggccc ttgaagagga
      481 cctgggagta gatgaggtac aggccctctg atggcaccac cagctggtta tctctcagct
      541 ccacgccatt ggccaggagg gcattggccc ggcggttcag ccactggagc tgcccctcag
      601 cttgagggtt tgctacaaca tgggctacag gcttgtcact cggggttcga gaagatgatc
      661 tgactgcctg ggccagaggg ctgattagag agaggtccct ggggaactct tccctctggg
      721 ggccgatcac tccaaagtgc agcaggcaga agagcgtggt ggcgcctgcc acgatcagga
      781 aggagaagag gctgaggaac aagcaccgcc tggagccctg gggcccccct gtcttcttgg
      841 ggagcgcctc ctcggccagc tccacgtccc ggatcatgct t
//
