LOCUS       sequence-120279-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Ebp1 tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Wang GZ, Wolf D, Goff SP
  TITLE     EBP1, a novel host factor involved in primer binding site-dependent 
            restriction of moloney murine leukemia virus in embryonic cells.
  JOURNAL   J Virol. 2014 Feb;88(3):1825-9. doi: 10.1128/JVI.02578-13. Epub 2013
            Nov 13.
  PUBMED    24227866
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02578-13"; journalName: "J Virol"; date: "2014-02"; 
            volume: "88"; issue: "3"; pages: "1825-9"
COMMENT     Sequence Label: pCMV-HA-Ebp1
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          143..239
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             303..329
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatccggta ctagaggaac
      121 tgaaaaacca gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg
      181 atccggtggt ggtgcaaatc aaagaactgc tcctcagtgg atgttgcctt tacttctagg
      241 cctgtacgga agtgttactt ctgctctaaa agctgcggaa ttgtacccgc gggcccacca
      301 tgtacccata cgatgttcca gattacgctc ttatggccat ggaggcccga attcaaatgt
      361 cgggcgagga cgagcaacag gagcaaacta tcgctgagga cctggtcgtg accaagtata
      421 agatgggggg cgacatcgcc aacagggtac ttcggtcctt ggtggaagca tctagctcag
      481 gtgtgtcggt actgagcctg tgtgagaaag gtgatgccat gattatggaa gaaacaggga
      541 aaatcttcaa gaaagaaaag gaaatgaaga aaggtattgc ttttcccacc agcatttcgg
      601 taaataactg tgtatgtcac ttctcccctt tgaagagcga ccaggattat attctcaagg
      661 aaggtgactt ggtaaaaatt gaccttgggg tccatgtgga tggcttcatc gctaatgtag
      721 ctcacacttt
//
