LOCUS       sequence-120282-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 YWHAB
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(931..955)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtagttctct ccctccccag cgtctccttc gtctccctgg ttttccgatg
      241 tccacagagt gagattgtcc ctaagtaact gcatgatcag agtgctgtct ttataagact
      301 cttcattcag cgtatccaat tcagcaattg cttcatcaaa tgccgttttt gccaggctac
      361 aggccttttc aggagagttt agaatctcat agtaaaagac tgagaaattt agtgccagac
      421 caagacgaat tgggtgtgta ggctgcattt ctttcttact aatttcaaat gcttcctggt
      481 aagcctgctg ggagttcgac acagtggttt gtttgttgtc tccagatgcc acttcagaaa
      541 gatacctaaa ataatctcct ttcattttca agtagaacac cttactttct ggttgtgtag
      601 cattgggaat aagatatttg tccaacagct ccagaacatc attgcagatg tcctgcagtt
      661 ctgcctctat cttctcacgg tactctttgc ccatctgctg cttcttctca ttcctctctg
      721 ttttctgctc aatgctggag atgacacgcc aggaagagcg gcgggcgcct accacattct
      781 tgtaggcaac agagagcaga tttctctctt cgttggagag ttcatgcccc tgttctgtga
      841 ctgccttcat ggctgcagcc atatcatcat atcgctcagc ctgctcagcg agtttggctt
      901 tctgtaccag ctcactttta tccattgtca tgccaacttt tttgtacaaa cttgtcggac
      961 c
//
