LOCUS       sequence-120291-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FL-EKLF driven by SV40 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Soni S, Pchelintsev N, Adams PD, Bieker JJ
  TITLE     Transcription factor EKLF (KLF1) recruitment of the histone 
            chaperone HIRA is essential for beta-globin gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13337-42. doi: 
            10.1073/pnas.1405422111. Epub 2014 Sep 2.
  PUBMED    25197097
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1405422111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-09-16- 16"; volume: "111"; issue: "37"; pages: 
            "13337-42"
COMMENT     Sequence Label: pSG5-FLAG-mEKLF
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          69..641
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     649..668
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     696..715
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        696..714
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      723..732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             732..755
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagtgagagg cttttttgga ggcctaggct tttgcaaaaa gctccggatc gatcctgaga
       61 acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca
      121 tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt
      181 atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat
      241 tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt
      301 tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat
      361 cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa
      421 caattgttat aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg
      481 gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat
      541 ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc
      601 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
      661 ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat
      721 tcgccaccat ggactacaag gacgacgatg acaaagatcc catggcctca gctgagactg
      781 tcttaccctc catcagtaca ctcaccaccc tgggacagtt tctggacacc caggaggact
      841 tcctca
//
