LOCUS       sequence-120299-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: NPFF2-Tango
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg aatagtttct ttggcacacc cgctgcgtca tggtgcctgc
      301 ttgagtctga cgtttccagt gctcctgata aggaggccgg gagagagcgc agggccctta
      361 gtgtgcaaca gagaggtgga ccagcatgga gtggttcact tgagtggtcc aggcagagcg
      421 ctggcgatag gcgacgcctg gggctgtccc gacaaacggc gaagagctcc tggtccagaa
      481 gcagggacag aacatgttgt tgccggcggg cctggtggat tctcgttcct gctgctgata
      541 gggccagacg agaaagattt attatgaatg aaaagtggga taccaattct agtgaaaact
      601 ggcacccaat ctggaatgtg aatgacacaa agcatcatct ttattccgat attaatataa
      661 cgtatgtaaa ctattatctg catcagccac aagtggccgc tatctttatc atcagctact
      721 ttctgatttt ctttctttgc atgatgggaa acaccgttgt ttgcttcatc gtgatgcgaa
      781 ataagcatat gcataccgtg accaacttgt ttatcctgaa cttggcaatc agtgatttgc
      841 tcgtaggaat attctgcatg cccattactc tgctcgataa tattatcgca gggtggccct
      901 tcggaaacac
//
