LOCUS       sequence-120320-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: MC2R-Tango
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg aagcatatca tcaattccta tgagaatatc aataacacgg
      301 ccaggaacaa tagcgactgt cccagggtag ttctgcctga agagatattc ttcaccatat
      361 ccatagtggg cgtactggaa aacctcatcg ttttgctggc ggtctttaag aataagaacc
      421 tgcaggctcc aatgtacttt tttatttgct ccctggccat tagcgatatg ctggggagcc
      481 tctataagat cctggagaac atccttataa ttttgcggaa catgggttac ctcaaacctc
      541 gcggatcttt cgaaaccacc gccgatgata tcatagactc cctgtttgtc ctgtccttgc
      601 tgggctcaat tttcagcctg agcgttattg ccgccgaccg ctacattaca atcttccacg
      661 cccttcgcta tcactccatc gtgactatga gacggacagt tgtggtactc acggtcattt
      721 ggactttttg taccggcact ggaatcacca tggtcatctt cagtcaccac gtcccaacgg
      781 ttattacgtt cacatccctg ttccctctga tgctggtctt cattctctgt ctgtacgtac
      841 acatgttcct gctcgcccgg tcccatacca ggaagatatc cacgttgccc cgagctaaca
      901 tgaagggagc catcaccctt accattctgc tgggtgtctt catcttttgc tgggctccct
      961 ttgtgctcca cgtgctgttg atgaccttc
//
