LOCUS       sequence-120335-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFAM
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg caaacactcc tcagcaccat attttcgttg tttctttatt gtgcgacgta
      241 gaagatcctt tcgtccaact tcaatcattt gttcttccca agacttcatt tcattatgat
      301 aacgagtttc gtcctcttta gcatgctgaa tatataattc cttttcagag tcagacagat
      361 ttttccagtt ttcctttaca gtcttcagct tttcctgcgg tgaatcaccc ttagcttctt
      421 ggaatctttc agctacataa acgttataag ctgaacgagg tctttttggt tttccaagca
      481 gtgttaactc tttttttttt gtcatagctt tcctttttaa atgtttgtcc atgatttctt
      541 tttccaaaga cataatctga cttggagtta gctgttcttt aaatctgcat atctcttctt
      601 tatatacctg ccactccgcc ctataagcat cttgatatat ttttttcttt gaatcaggaa
      661 gttccctcca acgttgggca attcttctaa ttagttctgt agtttttgca tctgggttct
      721 gagctttaaa tatgggtagt tgttctttag aaaatcgaag gtaagaactt acaggtttct
      781 ttggacaact tgccaagaca gatgaaaacc acctcggtaa atacacaaaa ctgaaggggg
      841 agcgcagtcg acttccacag ccggtgcaca gctctgctcc agaccttccc agggcactca
//
