LOCUS       sequence-120337-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_1_129
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             559..576
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(629..647)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      643..690
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcgcggat ccgaattcga gctccgtcga cccatgaaca acggcggcaa agccgagaag
      181 gagaacaccc cgagcgaggc caaccttcag gaggaggagg tccggaccct atttgtcagt
      241 ggccttcctc tggatatcaa acctcgggag ctctatctgc ttttcagacc atttaagggc
      301 tatgagggtt ctcttataaa gctcacatct aaacagcctg taggttttgt cagttttgac
      361 agtcgctcag aagcagaggc tgcaaagaat gctttgaatg gcatccgctt cgatcctgaa
      421 attccgcaaa cactacgact agagtttgct aaggcaaaca cgaagatggc caagaacaaa
      481 ctcgtaggga ctccaaaccc cagtactcct ctgcccaaca ctgtacctca gttcattgcc
      541 tgagcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      601 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      661 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      721 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      781 t
//
