LOCUS       sequence-120338-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_1_129
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(472..504)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(508..525)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(535..552)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             571..593
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    608..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(632..651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(633..651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg ctcaggcaat
       61 gaactgaggt acagtgttgg gcagaggagt actggggttt ggagtcccta cgagtttgtt
      121 cttggccatc ttcgtgtttg ccttagcaaa ctctagtcgt agtgtttgcg gaatttcagg
      181 atcgaagcgg atgccattca aagcattctt tgcagcctct gcttctgagc gactgtcaaa
      241 actgacaaaa cctacaggct gtttagatgt gagctttata agagaaccct catagccctt
      301 aaatggtctg aaaagcagat agagctcccg aggtttgata tccagaggaa ggccactgac
      361 aaatagggtc cggacctcct cctcctgaag gttggcctcg ctcggggtgt tctccttctc
      421 ggctttgccg ccgttgttca tgggtcgacg gagctcgaat tcggatccgc gacccatttg
      481 ctgtccacca gtcatgctag ccatatggct gccgcgcggc accaggccgc tgctgtgatg
      541 atgatgatga tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt
      601 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      661 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      721 tgcggtt
//
