LOCUS       sequence-120340-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ATF1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaaacactt ttattggaat aaagatcctt caaagttttt aactcttcta
      241 ttagagtttt attttgattt tccaggactg caactcggtt ttccaggcat ttcacatatt
      301 ctttcttctt tctgcgacat tctcgagcag cttctctgtt tttcattaac cttatttctc
      361 ttttcaattg ggggtcatct gtcttagttg tctgagaggt gagagtcaca ggagatgtca
      421 tcaccacagt ttgtggcaga gaagtagctg aaggtgtagt tcggatctga tatgtttgca
      481 tatctcctga tgcagtttgt acgaccacct gattgctggg cacaagtatc tgctgtccat
      541 cagaggtctg tgcatactga agaatagttg taccttgctg agtactgcct gaatttgtca
      601 tggttaatgt ctgaagtccc tgtactccat ctgtgcctgg acttgccaac tgtaaggctc
      661 catttggggc aatggcaata tactgtccgc tgctagtctg atagatggga gttggaacag
      721 acatagaagt gacagcagca gaaactccag aattttctcc gtctcctttt ctgccccgtg
      781 tatcttcaga agataagtct ttcaaaattt ttctgtaaga tgggcgccgt gctaggatcc
      841 cgtgggcttt ctgtgaggag cctatgctgt cggatgagtc ctgggactcc tcactttctg
//
