LOCUS       sequence-120343-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_1_144
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             90..122
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             603..620
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(673..691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      687..734
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tggctagcat gactggtgga cagcaaatgg
      121 gtcgcggatc cgaattcgag ctccgtcgac ccatgaacaa cggcggcaaa gccgagaagg
      181 agaacacccc gagcgaggcc aaccttcagg aggaggaggt ccggacccta tttgtcagtg
      241 gccttcctct ggatatcaaa cctcgggagc tctatctgct tttcagacca tttaagggct
      301 atgagggttc tcttataaag ctcacatcta aacagcctgt aggttttgtc agttttgaca
      361 gtcgctcaga agcagaggct gcaaagaatg ctttgaatgg catccgcttc gatcctgaaa
      421 ttccgcaaac actacgacta gagtttgcta aggcaaacac gaagatggcc aagaacaaac
      481 tcgtagggac tccaaacccc agtactcctc tgcccaacac tgtacctcag ttcattgcca
      541 gagagccata tgagctcaca gtgcctgcac tttaccccag tagctgagcg gccgcactcg
      601 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      721 tgaggggttt tttgctg
//
