LOCUS       sequence-120344-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_1_144
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(514..546)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(550..567)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(577..594)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             613..635
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    650..674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(674..693)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(675..693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgctc agctactggg
       61 gtaaagtgca ggcactgtga gctcatatgg ctctctggca atgaactgag gtacagtgtt
      121 gggcagagga gtactggggt ttggagtccc tacgagtttg ttcttggcca tcttcgtgtt
      181 tgccttagca aactctagtc gtagtgtttg cggaatttca ggatcgaagc ggatgccatt
      241 caaagcattc tttgcagcct ctgcttctga gcgactgtca aaactgacaa aacctacagg
      301 ctgtttagat gtgagcttta taagagaacc ctcatagccc ttaaatggtc tgaaaagcag
      361 atagagctcc cgaggtttga tatccagagg aaggccactg acaaataggg tccggacctc
      421 ctcctcctga aggttggcct cgctcggggt gttctccttc tcggctttgc cgccgttgtt
      481 catgggtcga cggagctcga attcggatcc gcgacccatt tgctgtccac cagtcatgct
      541 agccatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat gatggctgct
      601 gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      661 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcc
//
