LOCUS       sequence-120350-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: HTR5-Tango
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgcc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      121 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg cgccaccatg
      181 aagacgatca tcgccctgag ctacatcttc tgcctggtat tcgccgacta caaggacgat
      241 gatgacgcca gcatcgatat ggacctgccc gtcaacttga cgagtttcag tctgtccact
      301 ccaagtcctc ttgagactaa tcactctctt ggaaaggacg acttgaggcc ttccagccct
      361 ctcctctccg tgtttggggt gctgattctt accctgctcg gatttctggt agccgctact
      421 ttcgcatgga acctgttggt gctggcaacc attctccgcg tgcggacatt ccacagggtt
      481 ccccataatc ttgtggcatc catggcagtg agtgacgtac tggtagcagc actggtgatg
      541 cctctttctc tcgtccatga actcagcggg cggagatggc agcttggacg aagactttgt
      601 cagttgtgga ttgcatgtga cgtactttgc tgtactgctt ccatttggaa tgtcaccgcc
      661 attgcccttg accgctactg gtccatcact cgccatatgg agtatacctt gaggacccga
      721 aaatgtgtct ccaatgttat gatcgctctg acctgggccc tgagcgctgt gatcagcctt
      781 gcgccgctgc ttttcggatg gggagagaca tattcagagg gatcagagga atgccaggtc
      841 agtcgggagc cttcctatgc ggtgtttagc actgtcggcg ctttttatct cccactg
//
