LOCUS       sequence-120357-         414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conditional gene targeting vector; contain cloning sites for 5′ and 
            3′ homology arms, and a central targeting region flanked by LoxP 
            sites, and a neomycin cassette flanked by FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 414)
  TITLE     pEZ-Frt-lox-DT
REFERENCE   2  (bases 1 to 414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEZ-Frt-lox-DT
FEATURES             Location/Qualifiers
     source          1..414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..118
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     110..127
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(217..240)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
       61 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      121 ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctccaat cgaattctac
      181 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
      241 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
      301 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
      361 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaa
//
