LOCUS       sequence-120360-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conditional gene targeting vector; contain cloning sites for 5′ and 
            3′ homology arms, and a central targeting region flanked by LoxP 
            sites, and a neomycin cassette flanked by FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  TITLE     pEZ-Frt-lox-DT
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEZ-Frt-lox-DT
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    99..147
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(179..226)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    261..294
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    350..574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     563..580
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          648..713
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
       61 ccttctatcg ccttcttgac gagttcttct gaggggatcg gcaataaaaa gacagaataa
      121 aacgcacggg tgttgggtcg tttgttcgga tccgtcgagg gagcttcaaa agcgctctga
      181 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga tccccctggc
      241 gaaaggggga tgtggtcgac ataacttcgt ataatgtatg ctatacgaag ttataagctt
      301 gcggccggcc gccacctcga gccccagctg gttctttccg cctcagaagc catagagccc
      361 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
      421 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
      481 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
      541 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagga
      601 tctgcattcc accactgctc ccattcatca gttccatagg ttggaatcta aaatacacaa
      661 acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt taccttagag
      721 ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta aggttccttc
      781 acaaagagat cgcctgacac gatttcctgc acaggcttga gccatatact catacatcgc
      841 atcttggcca cgttttccac gggtttcaaa attaatctca agttctacgc ttaacgcttt
      901 cgcctgttcc cagttattaa tatattcaac gctagaactc ccctcagcga agggaaggct
      961 gagcactaca cgcgaagcac catcaccgaa ccttttgata aactcttccg ttccgacttg
//
