LOCUS       sequence-120364-         455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conditional gene targeting vector; contain cloning sites for 5′ and 
            3′ homology arms, and a central targeting region flanked by LoxP 
            sites, and a neomycin cassette flanked by FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 455)
  TITLE     pEZ-Frt-lox-DT
REFERENCE   2  (bases 1 to 455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEZ-Frt-lox-DT
FEATURES             Location/Qualifiers
     source          1..455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    95..128
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(157..204)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     269..291
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     283..300
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     284..300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgtcgaga
       61 tctagatatc gatggccata gcggccgcgg atccataact tcgtataatg tatgctatac
      121 gaagttatcc tttcgaggga gcttcaaaag cgctctgaag ttcctatact ttctagagaa
      181 taggaacttc ggaataggaa cttcaagatc cccctggcga aagggggatg tgctgcaagg
      241 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
      301 gaattcgagc tctcccatat ggtcgagcag tgtggttttc aagaggaagc aaaaagcctc
      361 tccacccagg cctggaatgt ttccacccaa tgtcgagcag tgtggttttg caagaggaag
      421 caaaaagcct ctccacccag gcctggaatg tttcc
//
