LOCUS       sequence-120369-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..714
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggtga gtgcgattaa gccagacatg
       61 aggatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      121 ggtacaggca agccttatga gggaaaacag accatggatc ttgaagtcaa agagggcgga
      181 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      241 gtgaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
      301 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca atgccagaaa cgacataaca
      361 atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat
      421 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
      481 cgtgatggag tgctgacggg tgatattgag atggctttgt tgcttgaagg aaatgcccat
      541 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
      601 gcccactttg tggaccacgc cattgagatt ttaagccatg acaaagatta caacaaggtt
      661 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
      721 ctcagatctg gcagcggtgg aggcttggag gagccggagg ccgcgacgcg gacagccgcg
      781 gccgtggatt gcaaagacag acccgggttc ccagtgaaga gg
//
