LOCUS       sequence-120378-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TP53
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacgagctat acaagctgga ggtgctcttc cagggtccga caagtttgta caaaaaagtt
       61 ggcatggagg agccacagtc agatcctagc gtcgagcccc ctctgagtca ggaaacattt
      121 tcagacctat ggaaactact tcctgaaaac aacgttctgt cccccttgcc gtcccaagca
      181 atggatgatt tgatgctgtc cccggacgat attgaacaat ggttcactga agacccaggt
      241 ccagatgaag ctcccagaat gccagaggct gctccccgcg tggcccctgc accagcagct
      301 cctacaccgg cggcccctgc accagccccc tcctggcccc tgtcatcttc tgtcccttcc
      361 cagaaaacct accagggcag ctacggtttc cgtctgggct tcttgcattc tgggacagcc
      421 aagtctgtga cttgcacgta ctcccctgcc ctcaacaaga tgttttgcca actggccaag
      481 acctgccctg tgcagctgtg ggttgattcc acacccccgc ccggcacccg cgtccgcgcc
      541 atggccatct acaagcagtc acagcacatg acggaggttg tgaggcgctg cccccaccat
      601 gagcgctgct cagatagcga tggtctggcc cctcctcagc atcttatccg agtggaagga
      661 aatttgcgtg tggagtattt ggatgacaga aacacttttc gacatagtgt ggtggtgccc
      721 tatgagccgc ctgaggttgg ctctgactgt accaccatcc actacaacta catgtgtaac
      781 agttcctgca tgggcggcat gaaccggagg cccatcctca ccatcatcac actggaagac
      841 tccagtggta atctactggg acggaacagc tttga
//
