LOCUS       sequence-120381-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag 
            and driven by SV40 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Chen X, Bieker JJ
  TITLE     Unanticipated repression function linked to erythroid Kruppel-like 
            factor.
  JOURNAL   Mol Cell Biol. 2001 May;21(9):3118-25.
  PUBMED    11287616
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2001-05"; volume: "21(9)"; pages: "3118-25"
COMMENT     Sequence Label: pSG5-FLAG-mEKLF Zinc Fingers
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          70..642
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     650..669
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     697..716
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        697..715
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     722..738
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     722..738
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      740..749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             749..772
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
       61 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      121 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      181 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      241 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      301 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      361 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      421 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      481 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      541 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      601 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      661 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      721 ttctagaact agtggatccg ccaccatgga ctacaaggac gacgatgaca aagatccccc
      781 catggcctca gctagcaaga ggcaggcggc acatacgtgc gggcacgaaa gctgcgggaa
      841 gagctactcc aagagctcgc acctcaaggc gcacctgcgc acgcacacgg
//
