LOCUS       sequence-120392-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of A2A receptor with N terminal TAP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Bergmayr C, Thurner P, Keuerleber S, Kudlacek O, Nanoff C, 
            Freissmuth M, Gruber CW
  TITLE     Recruitment of a cytoplasmic chaperone relay by the A2A adenosine 
            receptor.
  JOURNAL   J Biol Chem. 2013 Oct 4;288(40):28831-44. doi: 
            10.1074/jbc.M113.464776. Epub 2013 Aug 21.
  PUBMED    23965991
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464776"; journalName: "J Biol Chem"; date: 
            "2013-10-4- 4"; volume: "288"; issue: "40"; pages: "28831-44"
COMMENT     Sequence Label: N-TAP A2A in pcDNA3
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             525..545
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             552..665
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattcatgg gcacccccgc agtcaccacc tacaagctgg
      181 tcattaacgg caagactctg aagggcgaga ccaccaccaa ggccgtggac gcagaaaccg
      241 cggaaaaggc gttcaagcag tacgcgaacg acaacggggt ggacggagtc tggacctacg
      301 atgacgctac aaaaacgttc accgtgaccg aggttaacac ccccgctgtg actacgtaca
      361 agctggtgat caatgggaag accctgaagg gcgagaccac cacgaaagct gtagacgccg
      421 agacagccga gaaggccttc aagcagtacg ccaatgacaa cggcgtggat ggcgtgtgga
      481 cctatgacga cgccacgaag acattcactg tgactgagat cgatgagaac ctctacttcc
      541 agggcggcag tatggacgag aagaccaccg gctggagagg cggccacgtg gtcgagggac
      601 tggccggtga gctggagcag ctacgggccc gccttgagca ccacccccag gggcagcgcg
      661 agccgggccg gccgatgccc atcatgggct cctcggtgta catcacggtg gagctggcca
      721 ttgctgtgct ggccatcctg ggcaatgtgc tggtgtgctg ggccgtgtgg ctcaacagca
      781 acctgcagaa cgtcaccaac tactttgtgg tgtcactggc ggcggccgac atcgcagtgg
      841 gtgtgctcgc catccccttt gccatcacca tcagcaccgg gttctgcgct gcctgccacg
      901 gctgcctctt cattgcctgc ttcgtcctgg tcctcacgca gagctccatc ttcagtctcc
      961 tgg
//
