LOCUS       sequence-120402-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of A2A receptor with N terminal TAP tag and C 
            terminal YFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Bergmayr C, Thurner P, Keuerleber S, Kudlacek O, Nanoff C, 
            Freissmuth M, Gruber CW
  TITLE     Recruitment of a cytoplasmic chaperone relay by the A2A adenosine 
            receptor.
  JOURNAL   J Biol Chem. 2013 Oct 4;288(40):28831-44. doi: 
            10.1074/jbc.M113.464776. Epub 2013 Aug 21.
  PUBMED    23965991
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464776"; journalName: "J Biol Chem"; date: 
            "2013-10-4- 4"; volume: "288"; issue: "40"; pages: "28831-44"
COMMENT     Sequence Label: N-TAP A2A-YFP pcDNA3
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             523..543
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             550..663
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attcatgggc acccccgcag tcaccaccta caagctggtc
      181 attaacggca agactctgaa gggcgagacc accaccaagg ccgtggacgc agaaaccgcg
      241 gaaaaggcgt tcaagcagta cgcgaacgac aacggggtgg acggagtctg gacctacgat
      301 gacgctacaa aaacgttcac cgtgaccgag gttaacaccc ccgctgtgac tacgtacaag
      361 ctggtgatca atgggaagac cctgaagggc gagaccacca cgaaagctgt agacgccgag
      421 acagccgaga aggccttcaa gcagtacgcc aatgacaacg gcgtggatgg cgtgtggacc
      481 tatgacgacg ccacgaagac attcactgtg actgagatcg atgagaacct ctacttccag
      541 ggcggcagta tggacgagaa gaccaccggc tggagaggcg gccacgtggt cgagggactg
      601 gccggtgagc tggagcagct acgggcccgc cttgagcacc acccccaggg gcagcgcgag
      661 ccgggccggc cgatgcccat catgggctcc tcggtgtaca tcacggtgga gctggccatt
      721 gctgtgctgg ccatcctggg caatgtgctg gtgtgctggg ccgtgtggct caacagcaac
      781 ctgcagaacg tcaccaacta ctttgtggtg tcactggcgg cggccgacat cgcagtgggt
      841 gtgctcgcca tcccctttgc catcaccatc agcaccgggt tctgcgctgc ctgccacggc
      901 tgcctcttca ttgcctgctt cgtcctggtc ctcacgcaga gctccatctt cagtctcct
//
