LOCUS       sequence-12041-e        9013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van 
            Oudenaarden A, Jaenisch R
  TITLE     Direct cell reprogramming is a stochastic process amenable to 
            acceleration.
  JOURNAL   Nature. 2009 Dec 3. 462(7273):595-601.
  PUBMED    19898493
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Dec 3. 462(7273):595-601."
COMMENT     Sequence Label: FUW-TetO-mLIN28
FEATURES             Location/Qualifiers
     source          1..9013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    661..1249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(714..734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1132..1143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1252..1268)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1253..1269)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1774..1954
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1980..1997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1986..2210
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2256..2684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2343..2362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2553..2574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2693..2713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2698..3027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2878..3013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2940..2959
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3075..3122
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3141..3515
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3645..3766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3682..3701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3736..3755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3815..3831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3815..3831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3828..3850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3839..3855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3863..3869,3870..3887,3888..3893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3908..3929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4046..4063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4217..4805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4297..4316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4976..5767,5768..5836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5599..5618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5837..5941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6016..6035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6207..6586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6588..6786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6741..6761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6804..6984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7031..7156
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7649..7882
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8067..8111
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8409..8526
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(8579..8597,8598..8620,8621..8639,8640..8662,8663..8681,
                     8682..8704,8705..8723,8724..8746,8747..8765,8766..8788,
                     8789..8807,8808..8830,8831..8849)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        8882..8919
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8916..8940
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaattcatgg gctcggtgtc caaccagcag tttgcaggtg gctgcgccaa ggcagcggag
       61 aaggcgccag aggaggcgcc gcctgacgcg gcccgagcgg cagacgagcc gcagctgctg
      121 cacggggccg gcatctgtaa gtggttcaac gtgcgcatgg ggttcggctt cctgtctatg
      181 accgcccgcg ctggggtcgc gctcgacccc ccggtggacg tctttgtgca ccagagcaag
      241 ctgcacatgg aagggttccg aagcctcaag gagggtgagg cggtggagtt cacctttaag
      301 aagtctgcca agggtctgga atccatccgt gtcactggcc ctggtggtgt gttctgtatt
      361 gggagtgagc ggcggccaaa agggaagaac atgcagaagc gaagatccaa aggagacagg
      421 tgctacaact gcggtgggct agaccatcat gccaaggaat gcaagctgcc accccagccc
      481 aagaagtgcc acttttgcca aagcatcaac catatggtgg cctcgtgtcc actgaaggcc
      541 cagcagggcc ccagttctca gggaaagcct gcctacttcc gggaggaaga ggaagagatc
      601 cacagccctg ccctgctccc agaagcccag aattgagaat tcgatatcaa gcttatcgat
      661 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
      721 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      781 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      841 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
      901 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
      961 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     1021 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     1081 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     1141 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     1201 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     1261 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     1321 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     1381 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     1441 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     1501 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     1561 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     1621 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     1681 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     1741 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     1801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1921 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     1981 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     2041 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     2101 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     2161 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     2221 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     2281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2401 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2461 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2521 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2581 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2641 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     2701 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2761 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     2821 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2941 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     3001 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     3061 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     3121 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     3181 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     3241 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     3301 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     3361 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     3421 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     3481 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     3541 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     3601 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     3661 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3721 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     3781 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3961 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5281 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5581 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5941 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     6001 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     6061 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     6121 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     6181 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     6241 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6301 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6361 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6421 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6481 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6541 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6601 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6661 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6721 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6781 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6841 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6901 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6961 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7021 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7081 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7141 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7201 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7261 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7321 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7381 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7441 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7501 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7561 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7621 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7681 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7741 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7801 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7861 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7921 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7981 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8041 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8101 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8161 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8221 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8281 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8341 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     8401 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8461 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8521 aattttcggg tttattacag ggacagcaga gatccagttt ggttatcgag tttaccactc
     8581 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     8641 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     8701 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     8761 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     8821 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
     8881 agtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
     8941 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     9001 ctccgcggcc ccg
//
