LOCUS       sequence-120410-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound 
            Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 140_pSKB3-GBD-LUMP
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cgattacgat
       61 atcccaacga ccgaaaacct ttacttccag ggccatatgg gcctgagcgc acaggacatc
      121 agccagccgc tgcagaacag cttcatccat accggccacg gcgacagcga tcctcgccat
      181 tgctggggct ttccggaccg catcgacgag ctgtacctgg gcaacggcag cggcagcgct
      241 agctttagag gtattgttca aggtcgtggt gttattcgat ccatttcaaa aagtgaagat
      301 agtcagcgac atgggattgc attccctgag ggtatgtttc aacttgttga tgtagatact
      361 gtaatgttag taaatggttg ttcattgaca gttgtccgta ttttaggcga tatggtgtac
      421 tttgatatcg accaagcatt gggtacgaca acatttgatg gattaaaaga aggcgatcag
      481 gtaaacctag agatccaccc taaatttggt gaagtggttg gccgtggtgg attaacagga
      541 aatattaaag gcactgcgct ggtcgcagca ata
//
