LOCUS       sequence-120413-        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial 
            targeting sequence compatible with any N1 vector. Internal 
            restriction sites enable modular subcloning of 1x, 2x, 3x or 4x 
            presequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  TITLE     Mitochondrial LOC8R Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: N1-LOC8R
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             33..44
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             72..146
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             168..242
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             264..338
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             360..434
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(575..592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(765..784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca aggccgcata
       61 agcttgccac catgagcgtg ctgacccccc tgctgctgag aggcctgaca ggctctgcta
      121 gaaggctgcc tgtgcctcga gccaagatcc acagcctggg ggatccaatg tccgtgctga
      181 cacctctgct gctgcgggga ctgaccggaa gtgctcggag actgccagtg cccagggcca
      241 agattcactc tctgggggat cccatgagtg tgctgactcc actgctgctg cgcggcctga
      301 ctggatcagc acgccgcctg ccagtgcctc gagctaagat tcattccctg ggcgacccta
      361 tgtctgtgct gacccctctg ctgctgaggg ggctgactgg atctgcaagg cggctgcctg
      421 tgccaagggc caaaatccat agtctgggcg acccaccggt ctgggcctca tgggccttcc
      481 tttcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa catggtcata
      541 gctgtttcct tgcgtattgg gcgctctccg cttcctcgct cactgactcg ctgcgctcgg
      601 tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg ccaggaaccg
      661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt g
//
