LOCUS       sequence-120423-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PSmOrange targeted to mitochondria using the synthetic LOC8R 
            presequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  TITLE     Mitochondrial LOC8R Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LOC8R-PSmOrange
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..156
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             178..252
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             274..348
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             370..444
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      472..481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttgccac catgagcgtg ctgacccccc tgctgctgag aggcctgaca
      121 ggctctgcta gaaggctgcc tgtgcctcga gccaagatcc acagcctggg ggatccaatg
      181 tccgtgctga cacctctgct gctgcgggga ctgaccggaa gtgctcggag actgccagtg
      241 cccagggcca agattcactc tctgggggat cccatgagtg tgctgactcc actgctgctg
      301 cgcggcctga ctggatcagc acgccgcctg ccagtgcctc gagctaagat tcattccctg
      361 ggcgacccta tgtctgtgct gacccctctg ctgctgaggg ggctgactgg atctgcaagg
      421 cggctgcctg tgccaagggc caaaatccat agtctgggcg acccaccggt cgccaccatg
      481 gtgagcaagg gcgaggagaa taatatggcc atcatcaagg agttcatgcg cttcaaggtg
      541 cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      601 ccctacgagg gctttcagac cgctaagctg aaggtgacca agggcggccc cctgcccttc
      661 gcctgggaca tcctgtcccc tctcttcacc tacggctcca aggcctacgt gaagcacccc
      721 gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      781 atgaactacg aggacggcgg cgtggtgacc gtgacccagg actcctcact gcaggacggc
      841 gagttcatct acaaggtgaa gatgcgcggc accaacttcc cctccgacgg ccccgtgatg
      901 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
      961 ctgaagggcg agatcaggat gaggctgaag ctgaaggacg gcggccacta cacctccgag
     1021 gtcaagacca cctacaaggc caagaagtc
//
