LOCUS       sequence-120425-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, hygromycin resistance for plant 
            selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201H Cas9
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagggtaggc gcggcttaat tgcttaaatt tactattttt ttactatatt atactcaacc
       61 caatgagcat aaagactgta aaatctcaaa ttcctgagaa gcatatttat cgatcccaca
      121 gacttgatag ttccataatc catacgctgc agccaaattg ctagtgtgtt gaacatttaa
      181 cacgtagaga actagaaaag atataaaact aagattgata tccaaaatag acgagaacaa
      241 taagcaaaaa ctcttagttt tgaaataaat caacaatccc gagggttgtc acatacatca
      301 aaaacgaaaa tccatatagc aaaaaaaact ctaaattacc gttcgacaaa aagagaaaac
      361 tgataggaca tttgctaaac attaaaatca aaatttggat caagccgaat tgatcccggt
      421 cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
      481 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
      541 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
      601 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
      661 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
      721 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctc
//
