LOCUS       sequence-120430-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: OPRK1-Tango
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg gacagcccca ttcaaatctt ccggggcgag cccggaccaa
      301 cctgcgctcc aagcgcttgc ctgccaccga attctagtgc atggttccca gggtgggcag
      361 agcccgatag caatggctca gccgggtccg aggatgcgca gcttgaaccc gctcacatct
      421 cacccgctat ccctgtcatt attacggcag tctactcagt tgttttcgtt gtgggcctgg
      481 ttggaaatag cctcgtgatg tttgtgatta tcaggtatac taaaatgaag accgccacga
      541 atatctacat ctttaacctg gctctggctg acgccttggt gaccaccact atgccatttc
      601 agtccaccgt ctatcttatg aactcttggc cattcggcga cgttctgtgc aagattgtca
      661 tttccattga ctattataat atgtttacat caatctttac gcttacaatg atgagcgtgg
      721 accgatacat tgctgtttgc catccggtga aagcactgga ttttcgcacc cccttgaagg
      781 ccaagattat caatatctgt atttggctcc tgtcctcatc cgtcggaatt tccgccattg
      841 tcttgggtgg tacaaaggtg agggaggacg tggacgttat agagtgcagc ctgcagttcc
      901 ctgac
//
