LOCUS       sequence-120443-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET-30-based vector dedicated to efficient scFv expression, which 
            circumvents the problem of in-frame amber codons in scFvs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Ossysek K, Uchanski T, Kulesza M, Bzowska M, Klaus T, Wos K, Madej 
            M, Bereta J
  TITLE     A new expression vector facilitating production and functional 
            analysis of scFv antibody fragments selected from Tomlinson I+J 
            phagemid libraries.
  JOURNAL   Immunol Lett. 2015 Jul 25. pii: S0165-2478(15)30011-0. doi: 
            10.1016/j.imlet.2015.07.005.
  PUBMED    26219832
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Lett. 2015 Jul 25. pii: S0165-2478(15)30011-0. doi: 
            10.1016/j.imlet.2015.07.005."
COMMENT     Sequence Label: pET-scFv-T
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(175..630)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(312..333)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     524..543
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(667..714)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     710..728
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(781..798)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(826..843)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
       61 tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt
      121 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
      181 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
      241 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
      301 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
      361 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
      421 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      481 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      541 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
      601 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc aatccggata tagttcctcc
      661 tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctagttattg
      721 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatctca
      781 gtggtggtgg tggtggtgct cgaaggatcc gcgttattaa agcttgtgat ggtgatgatg
      841 atgtgcggcc gcccgtttga tttccacctt ggtcccttgg ccgaacgtat gaggccgctt
      901 caaaa
//
