LOCUS       sequence-120462-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ATF3
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..57
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(601..625)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatacaagc tggaggtgct cttccgggtc cgacaagttt gtacaaaaaa gttggcatga
       61 ttcttcaaca cccaggccag gtctctgcct cggaagtgag tgcttctgcc atcgtcccct
      121 gcctgtcccc tcctgggtca ctggtgtttg aggattttgc taacctgacg ccctttgtca
      181 aggaagagct gaggtttgcc atccagaaca agcacctctg ccaccggatg tcctctgcgc
      241 tggaatcagt cactgtcagc gacagacccc tcggggtgtc catcacaaaa gccgaggtag
      301 cccctgaaga agatgaaagg aaaaagaggc gacgagaaag aaataagatt gcagctgcaa
      361 agtgccgaaa caagaagaag gagaagacgg agtgcctgca gaaagagtcg gagaagctgg
      421 aaagtgtgaa tgctgaactg aaggctcaga ttgaggagct caagaacgag aagcagcatt
      481 tgatatacat gctcaacctt catcggccca cgtgtattgt ccgggctcag aatgggagga
      541 ctccagaaga tgagagaaac ctctttatcc aacagataaa agaaggaaca ttgcagagct
      601 acccaacttt cttgtacaaa gtggtgtaag cggccgccct cctcctcctt tcttgttcct
      661 ttcacgtcgc cttctcggtt gtagctggca gacgacgagt cttactttta cgtgtacttc
      721 tctatagatg atgtatgatc tctctgcatg cgtgttcgtg catgtgtccg tgtgttgggt
      781 acgcgtggta ccc
//
