LOCUS       sequence-120463-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BTF3
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(545..569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctatacaag ctggaggtgc tcttccgggt ccgacaagtt tgtacaaaaa agttggcatg
       61 aaagaaacaa tcatgaacca ggaaaaactc gccaaactgc aggcacaagt gcgcattggt
      121 gggaaaggaa ctgctcgcag aaagaagaag gtggttcata gaacagccac agcagatgac
      181 aaaaaacttc agttctcctt aaagaagtta ggggtaaaca atatctctgg tattgaagag
      241 gtgaatatgt ttacaaacca aggaacagtg atccacttta acaaccctaa agttcaggca
      301 tctctggcag cgaacacttt caccattaca ggccatgctg agacaaagca gctgacagaa
      361 atgctaccca gcatcttaaa ccagcttggt gcggatagtc tgactagttt aaggagactg
      421 gccgaagctc tgcccaaaca atctgtggat ggaaaagcac cacttgctac tggagaggat
      481 gatgatgatg aagttccaga tcttgtggag aattttgatg aggcttccaa gaatgaggca
      541 acctgcccaa ctttcttgta caaagtggtg taagcggccg ccctcctcct cctttcttgt
      601 tcctttcacg tcgccttctc ggttgtagct ggcagacgac gagtcttact tttacgtgta
      661 cttctctata gatgatgtat gatctctctg c
//
