LOCUS       sequence-120464-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFEC
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..43
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    44..68
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catggacgag ctatacaagc tggaggtgct cttccagggt ccgacaagtt tgtacaaaaa
       61 agttggcatg acccttgatc atcagatcat caatccaact cttaaatggt cacaacctgc
      121 agtgccaagt ggtgggcctc ttgtgcagca tgcacacaca actctggaca gtgatgctgg
      181 cctcacagaa aacccactca ccaagttact agctattggg aaagaagatg acaatgcaca
      241 atggcattta tctggaagta ttttggatgt gtatagcggt gaacaaggaa tttcaccaat
      301 taacatgggg cttacaagtg cttcttgtcc aagtagtcta ccaatgaaaa gagaaattac
      361 agaaactgac actagagctt tagcaaaaga gagacaaaaa aaggacaacc acaacctcat
      421 tgaaagaaga agaaggtata atattaatta ccgaatcaag gagcttggca ctcttattcc
      481 aaagtctaat gatcctgata tgcgctggaa caaaggaacc attctaaaag catcagtgga
      541 gtacatcaag tggctacaaa aagaacaaca gagagcccga gaattggaac acagacagaa
      601 gaaattagag caggctaaca ggcgacttct ac
//
