LOCUS       sequence-120468-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 YWHAB
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..34
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(798..822)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctatacaag ctggaggtgc tcttccaggg tccgacaagt ttgtacaaaa aagttggcat
       61 gacaatggat aaaagtgagc tggtacagaa agccaaactc gctgagcagg ctgagcgata
      121 tgatgatatg gctgcagcca tgaaggcagt cacagaacag gggcatgaac tctccaacga
      181 agagagaaat ctgctctctg ttgcctacaa gaatgtggta ggcgcccgcc gctcttcctg
      241 gcgtgtcatc tccagcattg agcagaaaac agagaggaat gagaagaagc agcagatggg
      301 caaagagtac cgtgagaaga tagaggcaga actgcaggac atctgcaatg atgttctgga
      361 gctgttggac aaatatctta ttcccaatgc tacacaacca gaaagtaagg tgttctactt
      421 gaaaatgaaa ggagattatt ttaggtatct ttctgaagtg gcatctggag acaacaaaca
      481 aaccactgtg tcgaactccc agcaggctta ccaggaagca tttgaaatta gtaagaaaga
      541 aatgcagcct acacacccaa ttcgtcttgg tctggcacta aatttctcag tcttttacta
      601 tgagattcta aactctcctg aaaaggcctg tagcctggca aaaacggcat ttgatgaagc
      661 aattgctgaa ttggatacgc tgaatgaaga gtcttataaa gacagcactc tgatcatgca
      721 gttacttagg gacaatctca ctctgtggac atcggaaaac cagggagacg aaggagacgc
      781 tggggaggga gagaactacc caactttctt gtacaaagtg gtgtaagcgg ccgccctcct
      841 cctcctttct tgttcctttc acgtcgcctt ctcggttgta gctggcagac gacgagtctt
      901 acttttacgt gtacttctct atagatgatg tatgatctct ctgcatgcgt gtt
//
