LOCUS       sequence-120470-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFAM
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..63
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(805..829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacgagctat acaagctgga ggtgctcttc cgggtccgac aagtttgtac aaaaaagttg
       61 gcaccatggc gtttctccga agcatgtggg gcgtgctgag tgccctggga aggtctggag
      121 cagagctgtg caccggctgt ggaagtcgac tgcgctcccc cttcagtttt gtgtatttac
      181 cgaggtggtt ttcatctgtc ttggcaagtt gtccaaagaa acctgtaagt tcttaccttc
      241 gattttctaa agaacaacta cccatattta aagctcagaa cccagatgca aaaactacag
      301 aactaattag aagaattgcc caacgttgga gggaacttcc tgattcaaag aaaaaaatat
      361 atcaagatgc ttatagggcg gagtggcagg tatataaaga agagatatgc agatttaaag
      421 aacagctaac tccaagtcag attatgtctt tggaaaaaga aatcatggac aaacatttaa
      481 aaaggaaagc tatgacaaaa aaaaaagagt taacactgct tggaaaacca aaaagacctc
      541 gttcagctta taacgtttat gtagctgaaa gattccaaga agctaagggt gattcaccgc
      601 aggaaaagct gaagactgta aaggaaaact ggaaaaatct gtctgactct gaaaaggaat
      661 tatatattca gcatgctaaa gaggacgaaa ctcgttatca taatgaaatg aagtcttggg
      721 aagaacaaat gattgaagtt ggacgaaagg atcttctacg tcgcacaata aagaaacaac
      781 gaaaatatgg tgctgaggag tgtttgccaa ctttcttgta caaagtggtg taagcggccg
      841 ccctcctcct cctttcttgt tcctttcacg tcgccttctc ggttgtagct ggcagacgac
      901 gagtcttact tttacgtgta cttctctata gatgatgtat gatctctctg catgcgtgtt
      961 cgtgcatgtg tccgtgtgtt gggtaccc
//
