LOCUS       sequence-120475-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ATF1
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(875..899)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctataca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagttggc
       61 atggaagatt cccacaagag taccacgtca gagacagcac ctcaacctgg ttcagcagtt
      121 cagggagctc acatttctca tattgctcaa caggtatcat ctttatcaga aagtgaggag
      181 tcccaggact catccgacag cataggctcc tcacagaaag cccacgggat cctagcacgg
      241 cgcccatctt acagaaaaat tttgaaagac ttatcttctg aagatacacg gggcagaaaa
      301 ggagacggag aaaattctgg agtttctgct gctgtcactt ctatgtctgt tccaactccc
      361 atctatcaga ctagcagcgg acagtatatt gccattgccc caaatggagc cttacagttg
      421 gcaagtccag gcacagatgg agtacaggga cttcagacat taaccatgac aaattcaggc
      481 agtactcagc aaggtacaac tattcttcag tatgcacaga cctctgatgg acagcagata
      541 cttgtgccca gcaatcaggt ggtcgtacaa actgcatcag gagatatgca aacatatcag
      601 atccgaacta caccttcagc tacttctctg ccacaaactg tggtgatgac atctcctgtg
      661 actctcacct ctcagacaac taagacagat gacccccaat tgaaaagaga aataaggtta
      721 atgaaaaaca gagaagctgc tcgagaatgt cgcagaaaga agaaagaata tgtgaaatgc
      781 ctggaaaacc gagttgcagt cctggaaaat caaaataaaa ctctaataga agagttaaaa
      841 actttgaagg atctttattc caataaaagt gtttgcccaa ctttcttgta caaagtggtg
      901 taagcggccg ccctcctcct cctttc
//
